Journal of Dental Implants
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Year : 2011  |  Volume : 1  |  Issue : 1  |  Page : 7-12

Structural analysis of in situ biofilm formation on oral titanium implants

Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany

Correspondence Address:
Sebastian Grade
Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover
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Source of Support: The German Research Foundation (SFB 599, D8), Conflict of Interest: None

DOI: 10.4103/0974-6781.76425

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Background: The primary etiologic factor for peri-implant infections is the adhesion of biofilms on oral implant surfaces in the area of soft-tissue penetration. The aim of the present study was to examine in situ biofilm growth directly on implant-abutment surfaces without the use of oral splints and to determine the effect of intraoral abutment localization on biofilm growth. Materials and Methods: Fifteen titanium healing abutments were inserted in six patients for 14 days. The newly formed supragingival biofilm on the titanium surface of the healing abutments was stained with fluorescent Live/Dead Baclight kit before examination by confocal laser scanning microscopy. The biofilm was scanned in terms of its surface coverage and thickness and different sites. Results: The results show that the biofilm has a different structure in every patient, with the thickness of the biofilm structure ranging between 0 and 80 ΅m and the surface coverage between 0 and 97% of the abutment surface. There was similar biofilm surface coverage at different intraoral locations, whereas the biofilm was significantly thicker in the mandible as compared to maxillary implant abutments. Conclusion: The method uniquely describes an effective way to depict biofilm development on implant surfaces in the supra- and sub-gingival regions.

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